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Our findings demonstrated GSH reduction was linked with a decline in cognitive function as reported in the article Brain Glutathione Levels - A Novel Biomarker for Mild Cognitive Impairment and Alzheimer's Disease published by Biological Psychiatry

An aliquot of 5 l of this reaction was diluted to 180 l with reaction buffer (100 mM Tris (pH 8.0), 1.5 mM EDTA, 1 mM DTT) to attain final concentrations of 5 g ml 1 GSTO1 and 0.25 IC 50 of inhibitor