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The solvent was removed in a speed vac (5 h, 30 C, Concentrator Plus, Eppendorf), and the resulting dried peptides were stored at 20 C until analysis, described in Binding site identification isoDTB analysis section in the Supplementary Information

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For drug treatment, 10 M olaparib was added 1 h before the second release and 2 M Emetine (Tocris, 7342) was added 1 h before cell collection