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Peptide samples were analyzed with a high-energy collisional dissociation (HCD) fragmentation method with normalized collision energy at 25 and 41, acquiring one Orbitrap survey scan in the mass range of m/z 3002000 followed by MS/MS of the three most intense ions in the Orbitrap (R7500)

Furthermore, the mechanical rupture of the skin by microneedles has been shown to facilitate the fusing of liposomal bilayers with intercellular lipids, hence enhancing the release and diffusion of the active pharmaceutical agent [274]

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