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Increased melanin pigment results from a greater quantity of melanocytes within the nail matrix, which is known as melanocyte proliferation

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To reduce the number of steps in a sample preparation procedure a simultaneous reduction of disulfide bound with tris(2-carboxyethyl)phosphine (TCEP) and the selective pre-column derivatization of the thiol group with 1-benzyl-2-chloropyridinium bromide (BCPB) has been applied

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