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RNAscope ISH combined with Immunofluorescence RNAscope ISH targeting mouse Trpa1 and human TRPA1 , combined with UCN1 immunofluorescence were performed on mouse and human EWcp samples to confirm the expression of TRPA1 mRNA in human EWcp/UCN1neurons and to examine the effect of chronic alcohol consumption on Trpa1 expression and UCN1 content in the mouse EWcp

The duration varies depending on the underlying cause