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L-CN and GBB analyses were performed in MRM (Table 4), and each prepared sample was injected into ACQUITY HPLC BEH HILIC (1.7 um, 2.1 100 mm) column (Waters, 186003461, China) at a column temperature of 40 C for dissociation, and chromatography was performed using a gradient mobile phase consisting of water and 20 mM of ammonium formate (phase A) and acetonitrile (phase B) (Table 5)

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The cell viability was measured using an 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay kit (Sigma-Aldrich Corporation) following manufacturer guidelines

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