These proteins are: horse heart apocytochrome c (Stellwagen et al., 1972), protein phosphatase inhibitor-1 from rabbit skeletal muscle (19.2 kDa by sedimentation and 60.0 kDa by gel-filtration) (Nimmo and Cohen, 1978), a dopamine-and adenosine 3:5-monophosphate-regulated phosphoprotein from bovine caudate nucleus, DARPP-32 (27.6 kDa by sedimentation and 59 kDa by gel-filtration) (Hemmings et al., 1984), bovine cardiac troponin C in the absence of calcium (McCubbin and Kay, 1985), heat-stable microtubule-associated protein MAP2 (Hernandez et al., 1986), smooth muscle caldesmon (Lynch et al., 1987), brain-specific 14-kDa protein (14 kDa by Sodium Dodecyl Sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 57 kDa by gel filtration) (Nakajo et al., 1990), carboxyl-terminal transactivation domain of Vmw65 from herpes simplex virus type 1 (11.0 kDa from sedimentation and 55.0 kDa from gel-filtration) (Donaldson and Capone, 1992), prothymosin (Stokes radius is 1.77 larger than those expected for a compactly folded protein consisting of 109 amino acid residues) (Gast et al., 1995), chromogranin B (100 kDa by SDS-PAGE and 220 kDa by gel-filtration) (Yoo, 1995), human topoisomerase I (66 kDa by sedimentation and 300 kDa by gel-filtration) (Stewart et al., 1996), N-terminal domain of human topoisomerase I (198 residues, 66 kDa by sedimentation and 96 kDa by gel filtration) (Stewart et al., 1996), oncoprotein 18 (Op18)/stathmin from fetal calf thymus (17.22 kDa by mass-spectrometry and 64 kDa by gel-filtration) (Schubart et al., 1987
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BDNF protein levels were 32.16 (23.4736.59) ng/mL at an optimal dilution of 1:20 in the human free BDNF Quantikine ELISA (#DBD00, R&D Systems) (Table 2)
