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however, recent evidence suggests a prevalence of approximately 1 in 65 children [3], corresponding to an estimated 1.82 million affected children nationwide

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Sample type considerations Different biological matrices have different detection characteristics: Urine: Most common testing matrix, metabolites accumulate Blood/plasma: Shorter detection windows for most peptides Dried blood spots: Emerging method with good sensitivity Hair: Not commonly used for peptides but theoretically possible Most anti-doping testing uses urine