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The gut microbiota performs essential physiological functions by modulating the expression of genes involved in epithelial immune tolerance, providing vital nutrients to the host, and supporting the maturation and regulation of systemic immune responses (207)

[DOI] [PubMed] [Google Scholar] 60.Marengo B, de Ciucis C, Ricciarelli R, et al

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In short, a targeted PCR-based sequencing approach was used, where the V3V4 regions of the 16S rRNA gene were targeted to generate amplicons using the primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3) with the barcode