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The obtained supernatant was filtered through a nylon membrane with a pore size of 0.22 m, and it was injected into HPLC (Waters) equipped with a reversed phase column (250 mm 4.6 mm) Waters Spherisorb ODS-2 and 5 m particle size, coupled to an electrochemical detector (DECADE) fitted with a working carbon electrode and with a reference hydrogen electrode at 520 mV and with UV/Vis detector at 216 nm to detect GSSG
Costa, C., Soares, R
The aim of this work was to develop a fast, sensitive and simple method for the simultaneous determination of Cys, Hcy, GSH and N-Hcy-Lys in plasma samples that reduces the number of steps, shortens the total time of sample preparation and limits the amount of single-use polypropylene laboratory materials
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