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Total protein of the cells was extracted and reduced in the presence of 100 mmol/L DTT for 5 min on the boiling water bath, and subsequently alkylated with 50 mol/L iodoacetamide for 30 min at room temperature in the dark
In 2006, the research-and-development arm of Pliva was acquired by GlaxoSmithKline, which has ample resources for clinical trials, but the company has not commercialized the peptide
STC-1 cells were exposed to these test solutions for 30 min while again being equilibrated in a cell culture incubator
From cells to organs: the present and future of regenerative medicine
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