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After washing three times with TBST (Tris-buffered saline supplemented with 0.1% Tween-20), the samples were incubated for 1 hour at room temperature with a secondary antibody

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Ideal candidates for facial rejuvenation are generally healthy adults between 30 and 65 who notice one or more of the following: Men and women aged 30 to 65 who notice fine lines, wrinkles, or sagging skin developing over time