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AsGGT1 and AsGGT2 belonged to the subgroup containing Arabidopsis AtGGT4 that functions in the degradation of glutathione S -conjugates in the vacuole ( Arabidopsis AtGGT1 and AtGGT2 that function in the breakdown of extracellular glutathione ( FIGURE 2 Generally, in bacteria, yeast, plants, and mammals, GGT is a heteromeric protein consisting of large and small subunits, both of which are generated from a common inactive precursor polypeptide by autoprocessing ( AsGGT1 , AsGGT2 , and AsGGT3 possessed the conserved threonine residue required for autocatalytic processing and the amino acid residues necessary for GGT activity, which were previously identified by biochemical and structural analyses of GGTs from humans and E
